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The levels of alkaline phosphatase (ALP), BMP2, OCN and OPG gene expression in MSC, MSC-HMGB1, and MSC sheets-HMGB1 groups at days 3, 7, and 14 (A–D). The cells were stained with alizarin red S (ARS) on day 28 (E–G). Mineralization was quantified by the extraction of ARS-stained cells using 10% cetyl pyridinium chloride (CPC). (H) *indicates P < 0.05 vs. the MSC group and #indicates P < 0.05 vs. the MSC-HMGB1 group.
