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. 2017 Feb 2;8(26):42238–42251. doi: 10.18632/oncotarget.15034

Figure 1. Identification of MGAT5 as a key regulatory gene for anoikis resistance.

Figure 1

A. The relative anoikis resistance of four colon cancer cell lines and the relative transcription levels with a ratio of more than 2, when compared to that of WiDr were plotted (n=5). B. Calculation of Pearson correlation coefficient (r) identified MGAT5 as a key regulatory gene (r=0.932). C. Caspase-8 activation in the mock and WiDr:MGAT5 cells was compared by immunoblot analyses using an anti-cleaved caspase-8 antibody. D. The anoikis-related death was monitored by immunofluorescence using an anti-cleaved caspase-8 antibody. E. The early and late apoptotic deaths were monitored by Annexin V and propidium iodide staining, respectively, using a flow cytometer. The percent apoptotic cells were calculated from the flow cytometer results compared between mock and MGAT5-overexpressing cells (averages with S.E. for n=3). F. The MGAT5 transcription levels were measured by quantitative real time-PCR using colon cancer tissues and their normal counterparts. Relative mRNA levels were calculated from the Ct values for MGAT5 normalized by those for GAPDH. Colon cancer stages were divided into a lower Astler-Coller grades (A-B2) and higher ones (C1-D).