Figure 6. WEE1 protection of branching tube formation.

a. Fluorescence images of anti-CD31-labelled CLMECs (green) in tube formation on a bed of fibroblasts (the fibroblasts are not visible in the images). The co-cultures were treated daily for 5 days with 1 μM AZD1775 or its vehicle control (Control). Scale bars 800 μm. b. For experiments of the type exemplified in (a), mean data for tube length, number of branch points and tube surface area (n=3, N=9). c. Fluorescence images of confluent normal human dermal fibroblasts labelled with Vybrant® Dye Cycle™ (green) following incubation with 1 μM AZD1775 or its vehicle control (Control) for 5 days. Scale bars 400 μm. d. For experiments of the type exemplified in (c), mean number of fibroblasts per mm² (n=3, N=9). e. Fluorescence images of anti-CD31-labelled HUVECs (green) in tube formation on a bed of fibroblasts (the fibroblasts are not visible in the images). The co-cultures were treated daily for 5 days with 1 μM AZD1775 or its vehicle control (Control). Scale bars 800 μm. f. For experiments of the type exemplified in (d), mean data for tube length, number of branch points and tube surface area (n=3, N=9).