Skip to main content
. 2017 Mar 21;8(26):42382–42397. doi: 10.18632/oncotarget.16389

Figure 2. IK over-expression and activation with 1-EBIO had no effect on MDA-MB-231 proliferation, invasion, or migration.

Figure 2

(A) Proliferation of control and IK over-expressing MDA-MB-231 cells was quantified over 4 days for cells treated with vehicle control or 1-EBIO. There was no significant difference in cell number on any day (Day 4: p = 0.33, 1-way ANOVA). (B) Invasion through Matrigel coated transwell after 16 h using FBS as a chemoattractant. Average number of cells per field of view with 4 fields of view per sample and 4 replicates in each of 3 independent experiments. There was no significant difference in the number of invading cells across any condition (p = 0.32, 1-way ANOVA) (C) A confluent layer of MDA-MB-231 control or MDA-MB-231-IK was scratched and healing was assessed after 10 h with vehicle control or 1-EBIO treatment. There was no significant difference in the normalized surface area of wound recovery (p = 0.41, 1-way ANOVA). All data presented as mean with SEM of three independent replicates.