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. 2017 Apr 18;8(26):42466–42477. doi: 10.18632/oncotarget.17198

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Copyright: © 2017 Satoh et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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Cells were treated with pemetrexed (20 μM), cisplatin (40 μM), or cSBL (1 μM) for 0–72 h. The number of cells was estimated using a Muse^™ Count & Viability Kit. (A) Cell growth rates were calculated as the ratio of the cell number at 72 h to the cell number at 0 h and presented as a fraction of the controls. (B) The number of live and dead cells every 24 h is shown. Statistically significant differences in the live cell number at 72 h were observed in the treatment groups compared to the control (***). Statistically significant differences in the dead cell number at 72 h were observed in cSBL-treated cells compared to cisplatin- or pemetrexed-treated cells (^††). *p < 0.05, **p < 0.01, ***p < 0.001, ^††p < 0.01.

Cells were treated with pemetrexed (20 μM), cisplatin (40 μM), or cSBL (1 μM) for 0–72 h. The number of cells was estimated using a Muse^™ Count & Viability Kit. (A) Cell growth rates were calculated as the ratio of the cell number at 72 h to the cell number at 0 h and presented as a fraction of the controls. (B) The number of live and dead cells every 24 h is shown. Statistically significant differences in the live cell number at 72 h were observed in the treatment groups compared to the control (***). Statistically significant differences in the dead cell number at 72 h were observed in cSBL-treated cells compared to cisplatin- or pemetrexed-treated cells (^††). *p < 0.05, **p < 0.01, ***p < 0.001, ^††p < 0.01.