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. 2017 Mar 29;8(26):42602–42612. doi: 10.18632/oncotarget.16645

Figure 4. Annexin V targeted tumor vessels and inhibited tumor angiogenesis.

Figure 4

(A) Fluorescence images of tumor blood vessels. Vescular staining (immunofluorescent) was performed and showed co-localization of CD31 (red) and Desmin (green) in PBS-treated samples. Far few vescular endotheliums were detected in tumor sections treated with Annxin V (5.0 mg/kg and 10.0 mg/kg). (B) Annexin V targeted tumor vessels. Annexin V-EGFP was injected i.v. to mice bearing B16F10 solid tumors. After 30 min, frozen sections of tumor tissues were analyzed for the localization of Annexin V-EGFP and blood vessels identified by MECA-32 antibody. (C) In vitro endothelial cell wound healing assay. HUVEC cells treated with Annexin V migrated slower than its negative control in a dose-dependent manner. (D) Statistic analysis of HUVEC cell wound healing experiment (Mean ± SEM, n=3, **p<0.01 compared with negative control).