Figure 1. Overview of the NP-iPSC generation protocol and immunostaining of specific markers.
(A) NPCs transduced with SeV expressing human OCT3/4, SOX2, KLF4 and c-MYC. NPC derived iPS colonies emerge at 14–28 days after NP cell transduction. (B) Staining of Alkaline phosphatase (AP) and pluripotency markers (NANOG, OCT3/4, SOX2, TRA-1–60, TRA-1–81 and SSEA-4) in induced cell colonies