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. 2017 Apr 26;8(26):42700–42711. doi: 10.18632/oncotarget.17446

Figure 1. Overview of the NP-iPSC generation protocol and immunostaining of specific markers.

Figure 1

(A) NPCs transduced with SeV expressing human OCT3/4, SOX2, KLF4 and c-MYC. NPC derived iPS colonies emerge at 14–28 days after NP cell transduction. (B) Staining of Alkaline phosphatase (AP) and pluripotency markers (NANOG, OCT3/4, SOX2, TRA-1–60, TRA-1–81 and SSEA-4) in induced cell colonies