(A, B) Invasion and migration counts per field of PON1-overexpressing H460-PON1 and H1299-PON1 cells versus vector control cells for 24 h. (C, D) Invasion and migration counts per field of PON1-knocked down A549-shPON1 cells versus shControl cells for 24 h. (E) Scratch-wound healing ability of cells after pre-exposure to 0.01% DMSO, 10 mM NAC, or 10 mM NAC in combination with 10 μM Akt-inhibitor LY294002. Representative bar graphs show the width in μm of scratch recorded. Representative migration micrographs are shown taken using phase-contrast microscope. (F, G) Western blot analysis of whole cell lysates. Cells were treated with 0.01% DMSO, 10 mM ROS-inhibitor NAC, 1 mM ROS-stimulators H2O2 and 7 mM FeSO4. Cells were treated with or without combination of NAC and Akt-inhibitor LY294002 for G. All experiments were conducted at least in triplicate and biologically repeated at least twice. Statistical analysis was performed using unpaired Student's t test.