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. 2017 Apr 12;8(26):42817–42835. doi: 10.18632/oncotarget.17069

Figure 6. Glycolytic regulation controls PON1-dependent antioxidative response in lung cancer cells.

Figure 6

(A) Immunoblot detection of PON1 from whole cell lysates after glucose-enrichment in the media with indicated concentrations for 24 h. (B) Cells were serum-starved, glucose-starved, or glucose-enriched prior to Western blotting with PON1 antibody. (C) Homogenous detection of oxidized and reduced NAD+ and NADPH in H460-PON1, H1299-PON1, A549-shPON1, and their respective controls as indicated after the transfected cells have been exposed to the indicated glucose-deprivation periods. (D) ROS production after cells were either enriched with glucose or glucose-deprived. (E) Immunoblotting and immunoprecipitation of cells after glucose-enrichment or glucose-deprivation. Three independent experiments were summarized. Symbols represent mean ± S.E.M. n = 3~7; n.s., not significant; *P<0.05; **P<0.01.