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. 2017 Apr 19;8(26):42939–42948. doi: 10.18632/oncotarget.17224

Figure 2. Knockdown of E6AP restores p27 protein levels in vitro and in vivo.

Figure 2

sh-Ctrl and shE6AP transduced DU145 (A) and PC-3 (B) cell lines were treated with 0.2μg/mL Dox for 96hrs. Samples were analyzed for the expression of E6AP, p27 and HSP60 (loading control) by western blotting. Representative images of three independent experiments are presented here. (C) sh-Ctrl and shE6AP transduced DU145 cells were injected subcutaneously in NSG mice and treated with Dox as detailed in Material and Methods. Tumor samples were analyzed by western blotting for the expression of E6AP, p27 and HSP60 (loading control).