Figure 3. Prohibitin accumulates upon different stressing stimuli.

(A) Cells were treated with 25 μM of cisplatin (CIS) for 24 h, 200 μM of dacarbazin (DTIC) for 48 h or 800 μM of temozolomide (TEM) for 48 h or left untreated. As for Figure 3, protein extracts of melanoma cells under the different control and experimental conditions were analyzed for PHB accumulation, as compared to a protein loading control (beta-actin). R indicates the relative accumulation of PHB. (B) Prohibitin also accumulates under serum starvation. LB373 and SKMel 37 cell lines were cultured with (10%) or without (0%) fetal bovine serum (FBS) for 24 h. Prohibitin accumulated in both cell lines upon starvation (C). FBS deprivation induced ROS production, as measured by flow cytometry using the general oxidative stress indicator CM-H2DCFDA in LB373 and SKMel 37 cell lines. Representative experiment of three independent assays.