Table 1. Time course of differential protein accumulation by LB373 melanoma cells after treatment with cisplatin.
| Identification | Spot | Relative Spot Intensity (h) | ||
|---|---|---|---|---|
| 8 | 16 | 24 | ||
| 60 kDa Heat Shock Protein (Hsp60) | 48 | 1.2 | 1.3 | 3.3 |
| 50 | 3.2 | 3.5 | n.d. | |
| 52 | 3.1 | 2.6 | 1.8 | |
| 53 | 1.7 | 1.2 | 6.8 | |
| 67 | 0.1 | 0.5 | 0.1 | |
| 78 kDa glucose-regulated precursor (GRP78) | 39 | 2.0 | 1.4 | 4.6 |
| 156 | 0.4 | 1.4 | 4.0 | |
| 317 | 2.0 | 2.4 | 5.8 | |
| 361 | 0.6 | 0.9 | 2.0 | |
| Actin, cytoplasmic 1 (Beta-actin) | 146 | 3.5 | 6.0 | 4.1 |
| 147 | 2.9 | 4.3 | 3.0 | |
| 150 | 3.6 | 3.7 | 6.8 | |
| 152 | 3.7 | 3.3 | 5.0 | |
| Alpha enolase | 211 | 1.9 | 2.0 | 1.5 |
| ATP Synthase beta chain, mitochondrial precursor | 90 | 2.0 | 2.9 | 1.3 |
| 98 | 0.6 | 0.7 | 12.0 | |
| ATP Synthase D Chain, mitochondrial | 441 | 0.9 | 1.3 | 1.8 |
| Calreticulin Precursor | 120 | 0.9 | 1.4 | 0.3 |
| 479 | 0.4 | 0.4 | 0.8 | |
| Cathepsin D precursor | 328 | 0.7 | 2.2 | 1.9 |
| 329 | 0.5 | 1.1 | 1.9 | |
| Endoplasmic reticulum protein ERp29 precursor | 359 | 2.5 | 2.8 | 2.7 |
| Endoplasmin precursor | 45 | n.d. | n.d. | 8.2 |
| Eukaryotic translation initiation facotr 3 subunit 2 | 214 | 1.1 | 2.0 | 2.0 |
| 220 | 0.7 | 0.5 | 1.1 | |
| F-actin capping protein alpha-1 subunit | 250 | 1.2 | 2.7 | 2.3 |
| Galectin-1 | 514 | 0.8 | 1.0 | 1.9 |
| 517 | 0.7 | 1.3 | 2.4 | |
| Glutathione S-transferase P | 412 | 1.7 | 1.9 | 2.4 |
| Heat-Shock protein beta-1 | 370 | 2.7 | 2.4 | 0.5 |
| 375 | 2.2 | 4.6 | 3.3 | |
| Heat shock 70 kDa protein 1 (HSP70.1) | 183 | 1.8 | 2.8 | 1.7 |
| Inorganic pyrophosphatase | 279 | 2.7 | 2.9 | n.d. |
| Lamin A/C | 376 | 0.9 | 3.9 | 4.4 |
| Myosin light chain alkali, non-muscle isoform | 500 | 1.4 | 2.4 | 2.9 |
| Nucleophosmin | 221 | 0.3 | 4.1 | n.d |
| 222 | 1.5 | 3.2 | n.d | |
| 474 | 1.8 | 1.4 | 1.3 | |
| Nucleoside diphosphate kinase A | 498 | 0.4 | 0.3 | 0.7 |
| Peroxiredoxin 4 | 371 | 0.5 | 1.2 | 2.0 |
| Peroxiredoxin 6 | 383 | 2.0 | 1.1 | 1.4 |
| Phosphoglycerate kinase 1 | 396 | 0.8 | 0.5 | 1.1 |
| Prohibitin | 339 | 1.4 | 1.6 | 2.0 |
| Protein disulfide isomerase A3 precursor | 22 | 2.6 | 3.1 | 1.1 |
| 55 | 3.4 | 3.0 | 4.5 | |
| 57 | 3.5 | 3.7 | 2.6 | |
| 347 | 1.1 | 1.1 | 2.8 | |
| Rho GDP-dissociation inhibitor 1 | 384 | 0.9 | 1.6 | 2.3 |
| Stress-70 protein, mitochondrial precursor | 36 | 2.5 | 2.1 | 1.3 |
| 37 | 1.7 | 2.3 | 3.0 | |
| T-complex protein 1, epsilon subunit | 47 | 4.4 | 3.9 | 5.1 |
| Triosephosphate isomerase | 388 | 2.3 | 2.2 | 2.5 |
| 394 | 2.5 | 1.6 | 1.7 | |
| Tropomyosin alpha 3 chain | 310 | 5.4 | 3.6 | 3.4 |
| 312 | 4.6 | 3.7 | 1.7 | |
| Tubulin beta-5 chain | 286 | 0.5 | 0.3 | 0.3 |
| 292 | 0.9 | 1.0 | 0.4 | |
| Ubiquinol-cytochrome C reductase complex core protein 1 | 116 | 1.6 | 2.5 | 3.2 |
| Vimentin | 80 | 2.8 | 0.8 | n.d. |
| 93 | 1.7 | 1.0 | n.d. | |
| 154 | 0.9 | 1.1 | 4.1 | |
| 398 | 0.7 | 0.8 | 2.8 | |
Data are reported as the relative intensity of IEF-SDS-PAGE, CBB-250 stained gel spots after 8, 16 and 24h incubation with 25μM cisplatin, compared with untreated cells. Data for each time point are the average obtained for 3 gels. The Table lists 34 regulated proteins identified by MALDI-TOF which were detected as 62 isoelectric forms. n.d (not detected)