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A. Increased regulatory T cells in NSCLC-D and NSCLC-R; Decreased B. CD19+ B cells, C. CD4+ T cells, and D. CD4/CD8 ratio; Elevated E. CD8+ T cells and F. CD8+CD28- T cells. Abbreviations: NSCLC = non-small cell lung cancer; HV = healthy volunteers; NSCLC-D = NSCLC-at first diagnosis; NSCLC-R = NSCLC-at first relapse. *P < 0.05; ** P < 0.01; *** P < 0.001.
