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. Author manuscript; available in PMC: 2018 Jul 6.
Published in final edited form as: Mol Cell. 2017 Jun 8;67(1):84–95.e5. doi: 10.1016/j.molcel.2017.05.020

Figure 3. Autophagy deficiency causes Glut1 mis-sorting to late endolysosomes.

Figure 3

(A) atg5+/+ and atg5−/− MEFs exposed to hypoxia (H, 1% O2) for 24h and immunostained for Glut1 and LAMP1. (B) Pearson’s correlation coefficient for Glut1-LAMP1 colocalization (mean ±SEM, n=30 cells from 3 independent experiments). (C) Cells stably expressing GFP-Glut1 exposed to H for 24h or subject to acute glucose starvation (-glc) for 18h and probed for GFP and LAMP1. (D) Pearson’s correlation coefficient for GFP-LAMP1 colocalization (mean ±SEM, n=45 cells from 3 independent experiments). Cont, Control (full media, 20% O2); H, hypoxia (1% O2), -glc, glucose deficient media. P values calculated using ANOVA followed by Tukey’s HSD test. ***P<0.001, **P<0.01, *P<0.05; ns, not significant. Scale bars 10μm. Related to Figure S3.