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. 2017 Mar 3;8(25):40345–40358. doi: 10.18632/oncotarget.15875

Figure 2. Sensitivity and reproducibility of the DSTP.

Figure 2

A. Correlation between the expected and the observed VAF (%) for the FFPE Quantitative Multiplex control sample. Observed values are means of 3 independent experiments. B. Representation of the precision according to the read depth using serial dilutions of the FFPE Quantitative Multiplex control sample. C. For the Tru-Q NGS DNA 3 control sample, concordance between the percentages of VAF measured by droplet PCR (black bars) and by NGS using the DSTP in two independent laboratories (white and dashed bars). The results from each laboratory are expressed as the mean ± standard deviation (SD) of three independent experiments. D. Correlation between the expected and the observed VAF (%) for the Tru-Q NGS DNA 3 control sample. Observed values represent means of 6 independent experiments performed in the two laboratories. E. Using clinical samples extracted from FFPE, inter-laboratory reproducibility of 15 mutations present in the samples. F. Correlation between the VAF (%) reported by the two laboratories for the clinical samples.