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. 2017 Mar 23;8(25):40568–40582. doi: 10.18632/oncotarget.16501

Figure 3. Glibenclamide reduced radiosensitivity of mice hepatocyte line.

Figure 3

(A) NCTC-1469 cells were treated with glibenclamide (100μM) for 1 hour. 24 hour later cells viability was tested by CCK-8 assay to evaluate the glibenclamide toxcity. Pre-treated with glibenclamide (100μM) for 1 hour, cells viability under (B) different IR dose and (C) different time point were determined. (D) 10 days after radiation the clones were dyed with crystal violet and (E) were quantified. 48 hours after radiation cells were harvested to test apoptosis rate by Hoechst33342 staining (micrographs in F; quantified in G) and (H, I) flow cytometry. n=3, Data were presented as mean± SD. *P<0.05