Figure 6. Elimination of ROS wiped off the protective effect of glibenclamide on irradiated NCTC-1469 cells.

NCTC-1469 cells were pre-treated with glibenclamide (100μM) or glibenclamide plus NAC (500μM) for 1 hour. After been replaced medium cells received single 8Gy of irradiation. (C) 24 h later the cells viability were tested by CCK-8 assay (n=3) and (A, B) 48 h later the apoptosis rate was detected by flow cytometry (n=3). Data were presented as mean± SD. *P<0.05.