Figure 1. Chemosensitivity evaluation of resveratrol to T24 and EJ cells.

A. Effect of resveratrol treatment on human bladder cancer (HBC) T24 and EJ cells. Cells were incubated with different concentrations (0, 20, 40, 60, 80, 100, 150 and 200μM) resveratrol for different time periods (0, 6, 12, 24, 48 and 72h), respectively, and then the cells number was determined by MTT as described in the Materials and Methods. Data are presented as means ± S.D. of three independent experiments. Bars means standard errors, *P<0.05, **P<0.001 reveal significant difference between RV-treatment and Control HBC cells. ^#P<0.05, ^##P<0.001 show significant different between T24 RV-treatment cells and EJ RV-treatment cells. B. HE morphological staining performed on T24 and EJ cells without (Control) and with 100μM RV (Resveratrol) incubation for 48 hours (100×). Cells at a density of 4×10⁵ cells per well were placed in dishes with coverslips, then T24 and EJ cells were treated without (Control) and with (Resveratrol) 100μM resveratrol treatment for 48h. Cells coverslips were harvested for examination and T24 cells exhibited more obviously spindle-shaped change than EJ cells. C. Flow cytometry analysis on the fractionation of cell cycles and apoptotic cells in T24 and EJ cell populations without (Control) and with (Resveratrol) 100μM resveratrol incubation for 48 hours. Red arrows, indicate the peak of apoptotic cells. Data revealed a presentative experiment in triplicate with similar results.