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. 2017 Feb 2;8(25):40289–40304. doi: 10.18632/oncotarget.15041

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Copyright: © 2017 Yang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Representative HPLC/DAD analysis of resveratrol in T24 cells. (a) HBC T24 cells treated without RV as Control; (b, c) T24 cells treated with 100μM resveratrol for 48h, cell supernatant (b) and cell lysates (c) spiked with 1,8-dihydroxy anthraquinone (internal standard, IS). Peaks: M1. trans-resveratrol, t_R=13.82min; M2. cis-resveratrol, t_R=15.97min; M3. resveratrol monosulfate (RVS), t_R=6.67min; IS. 1,8-dihydroxy anthraquinone, t_R=25.93min (Internal standard/IS). B. Morphologic changes were evaluated by HE staining (100×), and T24 cells showed neither observable growth arrest nor morphological change until 24h resveratrol incubation. C & D. Quantification of RV and RVS in T24 cells. Resveratrol concentrations in the cell lysates and supernatant after 100μM resveratrol treatment for 3, 6, 12, 24 and 48h, respectively.