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. 2017 Mar 28;8(25):40741–40755. doi: 10.18632/oncotarget.16580

Figure 10. Effect of antioxidant NAC and cathepsin B inhibitor CA-074 Me in intestinal tissue injury and cathepsin B release in vivo.

Figure 10

BALB/c mice were pretreated with or without100mg/kg N-acetyl-L-cysteine (NAC, intraperitoneal) at 1h or 10mg/kg 50μM CA-074 Me (intraperitoneal) at 30min followed by exposure in a temperature-controlled chamber (ambient temperature 35.5±0.5°C and 60±5% relative humidity)until the rectal core temperature (Tc) reached 42°C. A. Pathological changes in the ileum of mice (magnification, ×200). B. Chiu scores of intestinal injury in mice. C. Cathepsin B was localized by immunohistochemical stain (magnification, ×200). D. Quantification of immunohistochemical stain for Cathepsin B. E. Expression of cathepsin B was determined by Western blot. β-actin was run as an internal control. F. Quantification of Western blots for cathepsin B.*P < 0.05, statistically significant relative to control (37°C),#P < 0.05, statistically significant relative to heat stress group (HS), (n=6/group).