Skip to main content
. 2017 Mar 28;8(25):40741–40755. doi: 10.18632/oncotarget.16580

Figure 8. Effect of antioxidant NAC on heat stress-induced lysosomal membrane permeabilization and cathepsin B release in SW480 Cells.

Figure 8

SW480 cells were pretreated with or without 2mMN-acetyl-L-cysteine (NAC) followed by incubation at43°C for 2h and further incubated at 37°C for 6h. A. Flow cytometry analysis heat stress-induced ROS. B. The histogram represents the quantification of ROS generation analyzed by flow cytometry. C. Confocal laser scanning microscopy images of fluorescently labeled cells (×400). D. Flowcytometry analysis to count cells with a reduced number of intact lysosomes (‘pale cells’). E. The histogram represents the quantification of number of ‘pale cells’ analyzed by flow cytometry. F. Localization of cathepsin B was visualized by immunofluorescence using a confocal microscope (x200). G. Expression of cathepsin B was determined by Western blot. β-actin was run as an internal control. H. Quantification of Western blots for cathepsin B. The data shown represent the mean ±SD of at least three independent experiments, performed in triplicate. *P < 0.05, statistically significant relative to control (37°C),#P < 0.05, statistically significant relative to heat stress group (HS).