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. 2017 May 23;8(25):39963–39977. doi: 10.18632/oncotarget.18102

Figure 2. PRMT5 is important for cell proliferation, anchorage-independent growth and migration of PDAC and CRC.

Figure 2

A. Western blot, confirming stable PRMT5 overexpression and shPRMT5 knockdown in PANC1 and HT29 cells. B. Cell proliferation assay, showing that cell proliferation was significantly higher in the WT-PRMT5 cell lines, while shPRMT5 cells exhibited quite opposite effect in both PANC1 and HT29 cell lines. C. Anchorage-independent growth assay, showing that anchorage-independent growth was significantly higher in the WT-PRMT5 cell lines, while dramatically reduced in the shPRMT5 cells. Both colony size and number are quantified at the bottom of the corresponding panels. The data represent the means ± standard deviation (S.D.) for three independent experiments. *P < 0.05 vs. control (Ctrl) group. D. Cell migration assay, showing that cell migration was significantly higher in the WT-PRMT5 overexpression cells, while significantly reduced in the shPRMT5 cells. Upper panels, representative pictures in 20X magnification. Lower panel, quantification for the change in migration. The data represent the means ± S.D. for three independent experiments. *P < 0.05 vs. Ctrl.