Figure 2. Effect of everolimus on CgA secretion in primary cultures of human pNETs.

(A) Primary culture cells from 3 pNETs (tumors 2,10,11) were incubated without (vehicle with 10⁶ fold DMSO dilution) and with 0.1-10 nM everolimus for 72 h and CgA secretion was measured as described in patients and methods. Results are expressed as mean ± SEM (n=3) in percent of control. Each assay was performed in triplicate. (°°) p<0.01 versus control. (B) CgA secretion determined as in A in primary culture cells from 19 pNETs incubated without (vehicle) and with 1 nM everolimus for 72 h. (C) CgA secretion determined in B of the 12 pNETs primary cultures that were responsive to 1nM everolimus treatment and CgA secretion of the 7 pNETs primary cultures that were nonresponsive to everolimus treatment. The horizontal bars represent the medians.