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. 2017 Apr 10;8(25):41044–41063. doi: 10.18632/oncotarget.17008

Figure 5. Effects of everolimus and the SSAs single treatments on the activity of the PI3K/Akt and MAPKinase ERK1/2 pathways.

Figure 5

Primary culture cells from pNETs were incubated 30 min (3 pNETs) or 24h in the absence ( vehicle) or in the presence of 1nM everolimus (Eve) (7 pNETs) (A, B) or 1nM octreotide (Oct) (5 pNETs) (C, D) or 1nM pasireotide (Pas) (5 pNETs) (E, F). The phosphorylated levels of p70S6K, Akt and ERK1/2 were analyzed by using western blotting as described in patients and methods. Representative immunoblots are given in (A), (C), (E). Means ± SEM in percent of control are given in (B), (D), (F) ((B) n=7; (D,F) n=5). Control condition (white bars), 30min treatment (hatched bars), 24h treatment (black and grey bars). (°) p<0.05, (°°°) p<0.001versus control.