Table 2. Molecular characteristics of pNET primary cultures.
| Tumor (number) | Grade | SST2R (copies/copies GUSβ) | CgA secretion | |
|---|---|---|---|---|
| Control (U/l) | octreotide (%inhibition) | |||
| 1 | G2 | 2.80 | 325 ± 9 | 28 ± 1** |
| 2 | G1 | 4.94 | 170 ± 5 | 8 ± 5 |
| 3 | G2 | 1.57 | 159 ± 1 | 49 ± 10* |
| 4 | G1 | 6.67 | 3941 ± 13 | 51 ± 4** |
| 5 | G2 | ND | ND | ND |
| 6 | G3 | 0.32 | 10 ± 1 | 44 ± 7* |
| 7 | G1 | 5.08 | 137 ± 6 | 93 ± 1*** |
| 8 | G2 | 0.96 | 49 ± 1 | 34 ± 11* |
| 9 | G2 | 1.62 | 40 ± 2 | 39 ± 2** |
| 10 | G2 | 0.50 | 488 ± 10 | 72 ± 1* |
| 11 | G2 | 0.53 | 21 ± 2 | 37 ± 6* |
| 12 | G2 | 2.23 | 514 ± 11 | 71 ± 1*** |
| 13 | G2 | 0.88 | 870 ± 125 | 63 ± 2** |
| 14 | G2 | 2.75 | 569 ± 48 | 16 ± 2* |
| 15 | G3 | 7.62 | 284 ± 13 | 93 ± 2** |
| 16 | G2 | 0.96 | 691 ± 87 | 76 ± 10* |
| 17 | G2 | 3.65 | 93 ± 19 | 47 ± 6* |
| 18 | G2 | 3.94 | 66 ± 2 | 36 ± 4** |
| 19 | G1 | 2.35 | 735 ± 33 | 36 ± 15* |
| 20 | G1 | ND | ND | ND |
| 21 | G1 | 3.23 | 16 ± 2 | 49 ± 9* |
| 22 | G1 | 2.67 | 568 ± 21 | 85 ± 1*** |
| 23 | G3 | 1.11 | ND | ND |
| 24 | G3 | 0.50 | ND | ND |
| 25 | G2 | 6.92 | ND | ND |
SST2 receptor mRNA was determined as described in patients and methods by real-time PCR. Cells were treated in the absence or in the presence of 1nM octreotide and CgA secretion was measured as described in patients and methods. (*) p<0.05; (**) p<0.01 (***) p<0.001 versus control conditions.