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. 2017 Mar 28;8(25):40791–40803. doi: 10.18632/oncotarget.16584

Figure 4. IL24-iMSCs induce in vitro melanoma cell apoptosis.

Figure 4

(A) ELISA analysis of IL24 in the supernatants from iMSCs and IL24-iMSCs is shown. IL24 levels in the supernatants of iPSCs, iMSCs and IL24-iMSCs were 6.33±0.97ng, 10.58±2.66ng and 45.18±2.53ng, respectively in 106 cells in 24h. (Data are mean ± SEM; n = 4 for each group; ns, p > 0.05; ****, p < 0.0001 by Oneway ANOVA). (B and C) Western blot analysis of cell lysates from control iMSCs and IL24-iMSCs. The expression levels of IL24 in IL24-iMSCs were about 2 fold higher than in control iMSCs.(Data are mean ± SEM; n = 3 for each group; ns, p>0.05, ***, p<0.001, ****, p<0.0001, One way ANOVA). (D and E) Flow cytometric analysis of cell apoptosis assay. B16-F10 cells co-cultured with IL24-iMSCs showed increased apoptosis compared to co-culturing with control iMSCs or control B16-F10 cells in vitro. (Data are mean ± SEM; n = 3 for each group; * p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, One way ANOVA).