Figure 6. The knockdown of vimentin inhibits oleate-induced tumor cell invasion.

(A and B) TU183 cells were transfected with 20 nM vimentin siRNA (siVIM) or scrambled oligonucleotides by lipofection. The invasive properties of the cells were examined using an invasion assay as described in the “Materials and Methods” section. The invading cells were fixed and stained with crystal violet and then examined using a microscope or the cells were solubilized with acetic acid, and the absorbance (OD, 595 nm) was measured in a microplate reader (A). The values are the mean ± s.e.m.. The expression of vimentin and β-actin was determined by Western blotting (B).