Figure 4.

The analysis of RVA zoonotic strain G4P[6]T7 in the study. (A) The genotype constellation of the case in investigation 16020_7 and other porcine RVA strains with the NSP3 T7 genotype. Details on dates of collection and age of host are given. The colour code in the host column is consistent with colour illustration of corresponding case in the map (panel B) in this figure. (B) The geographical location of the human case’s residency and pig farms that raised the pigs infected with RVA NSP3 T7 strains overlaid on the total sampling area (as shown in Supplementary Fig. S1). The colouring of the human case and pig farms is consistent with colour code presented in the column “Host” in panel of this figure. The red star indicates the Dong Thap Provincial Hospital where diarrhoeal patients were admitted. The map scale bar is shown in the units of geometric km. See Supplementary Fig. S1 for more information. (C) The time-resolved phylogenetic tree of RVA NSP3 T7 genotype sequences comparing local versus global sequences. Reference sequences were retrieved from GenBank [N = 69, excluded the duplicate sequence for TM-a strain (JX290174) and BP1901 (KF835960) which is 15 aa shorter than the complete ORF]. Strains were coloured according to the host species from which the strain was identified, and “VIZIONS” in the annotation refers to strains identified from this study. Sequences identified from this study were highlighted in red (human case 16020_7) or in turquoise (porcine sequences). The first T7 sequence identified (in a cow in 1973) was indicated in purple. Posterior probabilities of internal nodes with values ≥0.75 are shown and the scale axis indicates time in year of strain identification.