Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 21;7:6123. doi: 10.1038/s41598-017-06430-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

IL-12p70 and IL-18 levels do not correlate with higher MAIT cell responses in IVB compared to PB (a) Levels of IL-12p70 (top left, n = 4), IL-18 (top right, n = 4) and IL-10 (bottom left, n = 10) in culture supernatants from paired samples of PB, IVB, and DP following 16 hours of culture with or without E. coli stimulation. (b) Levels of IL-12p70 (left, n = 18) and IL-18 (right, n = 18) in paired plasma samples from PB and IVB measured with ELISA. (c,d) The effect of IFN-γ production in paired samples of PB and IVB following 16 hours of culture with E. coli stimulation after addition of indicated neutralizing antibodies. Data is expressed as percentage cytokine release compared to appropriate isotype control (black). (c) Flow cytometric analysis of intracellular MAIT cell expression of IFN-γ (anti-MR1 n = 5, anti-IL-12 n = 3, anti-IL-18 n = 2, anti-IL-12 + IL-18 n = 2, anti-MR1 + IL-12 + IL-18 n = 3). (d) Levels of IFN-γ measured with ELISA on culture supernatants (anti-MR1 n = 4, anti-IL-12 n = 4, anti-IL-18 n = 4, anti-IL-12 + IL-18 n = 3, anti-MR1 + IL-12 + IL-18 n = 4). Box and whisker plots show median, interquartile range and range of paired samples. In (a) and (b), the Wilcoxon signed rank test was used to detect statistically significant differences between paired unstimulated and stimulated samples (a), or two different groups (b), stars indicate significances. The Friedman test followed by the Wilcoxon signed rank test was used to detect statistically significant differences across multiple paired samples (a). A Bonferroni corrected p value was used for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001.