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. 2017 Jul 21;7:6123. doi: 10.1038/s41598-017-06430-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MR1 is expressed by macrophages in foetal villi, but not in syncytiotrophoblasts. Immunofluorescence microscopic images of term placental tissue showing cross sections of chorionic villi and intervillous space (IVS) stained with (a) MR1 (red) or (b) secondary antibody only (control) at magnification × 10. MR1 was expressed by cells inside the villi, but not in syncytiotrophoblasts. Sections of placental villi at (c–e) term and (g–i) gestation week 13 were stained for MR1 (red) and CD68 (green) (×20). (e and i) Merged images showed that MR1 and CD68 were coexpressed by cells in the villi. Sections stained only with secondary antibody (Cy3) and DyLight488-conjugated (D488) streptavidin complex were used as negative controls (f and j) (×20). Nuclei were stained with DAPI (blue). The same microscopy settings were kept for the samples and the corresponding controls, n = 2 for term placentas and n = 1 for 13 week gestation.