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. 2017 Jul 21;7:6123. doi: 10.1038/s41598-017-06430-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MR1 is expressed by decidual macrophages. (a) Immunofluorescence microscopic images of term placental membranes showing cross sections of decidua parietalis, chorion, and amnion at × 4 magnification. (b) MR1 staining in the outer decidual layer of the membrane and (c) secondary antibody only as control (×10). Decidual tissue stained with (d) MR1 antibody (red), (e) CD68 antibody (green), and (f) merged images showing coexpression of MR1 and CD68 (×40). (g) Sections stained only with secondary antibody (Cy3) and DyLight488-conjugated (D488) streptavidin complex were used as negative controls (×40). Nuclei were stained with DAPI (blue). The same microscopy settings were kept for the samples and the corresponding controls, n = 2.