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. 2017 Jul 21;7:6171. doi: 10.1038/s41598-017-06275-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of DMS treatment in cardiac function and parasite load. After an adaptation period in the treadmill chamber, naive and saline-treated or DMS-treated chronic Chagasic mice exercised at 5 different velocities (7.2, 14.4, 21.6, 28.8 and 36.0 m/min), with increasing velocity after 5 min of exercise at a given speed. (A) Distance run and (B) Time of exercise on a motorized treadmill. (C) Spleen fragments obtained from normal and T. cruzi-infected mice treated with saline or DMS were used for DNA extraction and qRT-PCR analysis for quantification of parasite load (primer 1 5′-GTTCACACACTGGACACCAA-3′ and primer 2 5′-TCGAAAACGATCAGCCGAST-3′). The standard curve of DNA ranged from 4.7 × 10⁻¹ to 4.7 × 10⁶). Bars represent means ± SEM of 8–10 mice/group. ***P < 0.001; **P < 0.01; *P < 0.05 compared to saline group; ^# P < 0.05 compared to naive group.