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. 2017 Jul 21;7:6171. doi: 10.1038/s41598-017-06275-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transmission electron micrographs of trypomastigotes treated or not with DMS for 24 h. (A) Untreated trypomastigotes presenting a typical morphology of the nucleus (N), kinetoplast (K), mitochondria (M) and Golgi complex (GC). (B,C) Trypomastigotes treated with DMS (1 µM) causes the formation of numerous and atypical vacuoles within the cytoplasm accompanied by a large loss of density. (D,E) Trypomastigotes treated with DMS (2 µM) shows degeneration of mitochondria and intense vacuolization. (F) Trypomastigotes treated with DMS (4 µM) shows myelin-figures. Black arrows indicate alterations cited. Scale bars: A = 1 µm; B–E = 0.5 µM; F = 0.2 µm.