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. 2017 Jul 21;7:6146. doi: 10.1038/s41598-017-06661-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of NTP on β-catenin localization in confluent HaCaT cells. (a) The results of FACS analysis using PI. HaCaT cells were seeded on 35 mm dish at the indicated concentration. Data shown are representative of four independent experiments. (b) The result of confocal imaging of immunofluorescence (Scale bar: 20 μm). Cells were treated with NTP for 5 minutes at 0, 2, 4, and 8 hours before harvesting. After fixation, cells were stained for E-cadherin (red) and β-catenin (green). DAPI was used for counter staining of the nuclei. nt: non-treated.