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. 2017 Jul 21;7:6146. doi: 10.1038/s41598-017-06661-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of NTP on β-catenin expression in confluent HaCaT cells. (a) The results of Western blot and RT-PCR. Confluent HaCaT cells were treated with NTP for 5 minutes at 3, 6, 9, 24 hours before harvesting. (b) Confluent HaCaT cells were treated with NTP for the indicated length of time and incubated for 6 hours. After harvesting the cells, the total protein extracts were used for Western blot analysis. GO: Gas only (argon). (c) One day after seeding, HaCaT cells were transfected with scrambled siRNA (sc) or siRNA against β-catenin. Six hours before harvesting, cells were treated, or not, with NTP for 5 minutes. After harvesting, the total RNA and protein extracts were used for RT-PCR and Western blot analysis, respectively. Data shown are representative of three independent experiments. GAPDH was used as loading control. All images presented here are the cropped images (see Supplementary information for the original images).