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. 2017 Jul 21;7:6146. doi: 10.1038/s41598-017-06661-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of NTP on wound healing in mouse skin. (a) Two equal-sized wounds were created in the dorsal skin of HRM2 mice, as described in the Materials & Methods section. One of the wounds was subjected to NTP treatment for 5 minutes, three times per week, for 2 weeks. Over the course of the treatments, external changes in the wounds were monitored by taking photographs at the indicated time points. Data shown are representative of four independent experiments. (b) On day 15, the skin tissue of the wounds were isolated, and subjected to H&E and trichrome staining. Data shown are representative of four independent experiments, and photographs were taken at 40X magnitude. Scale bar: 100 μm. (c) The results of IF assay against Ki-67 using the skin tissues. Scale bar: 20 μm.