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. 2017 Jul 21;7:6119. doi: 10.1038/s41598-017-06428-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Low-throughput synthesis of gene fragments. (a) Selective amplification of each subpool for generation of lycopene biosynthetic genes. Subpools were directly amplified using the oligo pool cleaved from the microchip as the template. (b) PCR of each error-depleted subpool. The oligo subpool was amplified with the error-depleted elute from etMICC as the template. (c) Fragments assembled by LCR from error-depleted oligo subpools. M, 20 bp DNA ladder (TaKaRa Bio). Red arrows indicate target fragments. OLS, oligo subpool; ER, error-removed.