Table 1.
Error analysis of assembled fragment sequences using different error-removal strategies with various throughputs
| Error type | Non-error removal | Low-throughput error removal | Throughput-improved error removal | Multiplex assembly | Double round error removala |
|---|---|---|---|---|---|
| Multi-errorb | 6 | 5 | 0 | 2 | 0 |
| Deletion | 175 | 7 | 4 | 4 | 1 |
| Insertion | 38 | 10 | 9 | 11 | 0 |
| Substitution | 229 | 27 | 23 | 29 | 4 |
| Total errors | 448 | 49 | 36 | 46 | 5 |
| Bases sequenced | 31445 | 23599 | 16343 | 24874 | 9243 |
| Error frequency (error per kb) | 14.25 | 2.08 | 2.20 | 1.85 | 0.54 |
| Ratio of error-free synthetic DNAc (%) | 3.23 | 48.44 | 41.51 | 55.5 | 81.48 |
aError-removal was conducted both at oligo and fragment stages.
bError site located on sequence that contains more than three adjacent consecutive nucleotide errors.
cLength of synthetic DNA was 319–423 bp for lycopene biosynthesis genes (without primer sequences).