Fig. 6.

ACLY is required for the establishment of H3atKO phenotype. a, b Immunofluorescence staining and quantification of ACLY, showing nuclear localization in Hdac3 knock out mice fed LFD (n = 3 per group), scale bar is 40 μm; c Morphology of cells infected with adenovirus expressing shRNA targeted to Hdac3 (shHDAC3), to Acly (shACLY) or both (shHDAC3 + shACLY) or to a scrambled control shRNA (scramble), scale bar is 50 μm; d Gene expression analysis in knock down cells (n = 3 per group); e H3K27ac ChIP analysis in knock down cells (n = 6 per group). Data are presented as mean ± SEM. Statistical analysis: One way ANOVA, Tukey as post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001 vs. scramble, ^§§ p < 0.01, ^§§§ p < 0.001 vs. shHDAC3