Fig. 7.

High fat diet overcomes the effects of adipose ablation of HDAC3. a Body weight gain of floxed and H3atKO mice fed HFD (n = 12–14 per group); b Glucose tolerance test of floxed and H3atKO mice (n = 12–14 per group), performed after 20 weeks of HFD; c Volcano plot of IngWAT metabolome in floxed and H3atKO mice fed HFD (n = 6 per group); d Heatmap of IngWAT metabolome in floxed and H3atKO mice fed HFD (n = 6 per group); e Hematoxylin–eosin staining of IngWAT from floxed and H3atKO mice fed HFD, scale bar is 100 μm; f Body temperature of HFD floxed and H3atKO mice exposed to 4 °C for 100 min (n = 8 per group); g, h Gene expression analysis of browning genes and lipolytic/lipogenic genes in IngWAT of floxed and H3atKO mice (n = 5 per group); i, j H3K27ac ChIP analysis showing lack of enrichment on Pparg and Ucp1 enhancers and on putative regulatory regions of Ppara gene in IngWAT from H3atKO HFD mice (n = 3 per group). Data are presented as mean ± s.e.m. Statistical analysis: Student’s t-test, *p < 0.05