Figure 5.
Inhibition of LcpK30 by carbon monoxide (CO). The cleavage of a polyisoprene latex emulsion (1 ml) was initiated by the addition of 4 µg of purified LcpK30 at t = 7 min (blue and red lines). The reaction was allowed to proceed until t = 14 min. At this time point, 200 µl of oxygen-saturated buffer (blue line) or 200 µl of a carbon monoxide-saturated buffer (red line) was added to the cuvettes. The dilution of the reaction buffer with the CO-buffer resulted in an immediate drop of the oxygen concentration. Note the continuous decrease of the oxygen consumption in the control (no CO, blue line). In contrast, the oxygen concentration did not decrease but increased in the CO-supplemented reaction (red line) due to diffusion from atmospheric oxygen.