Figure 5.

Characterization of the Cas9-mediated suppression of HBV replication. (A) HepG2.2.15.7 cells transduced with Cas9, nickase-Cas9 and d-Cas9 together with the pair of sgRNAs were collected at 5 days post-transduction, and the intracellular HBV RNA were determined by Northern blotting. (B) Total DNAs were extracted from HepG2.2.15.7 cells transduced with Cas9 and nickase-Cas9 together with the sgRNAs at 5 days post-transduction, and the target HBV sequences were directly sequenced after amplification. *p < 0.01 vs. the results for control cells. (full-length gels are presented in Supplementary Figure).