Table 3.

The primers used for the detection of the sequences of the possible off-target sites and HBV genome.

Off-target #1–1:

5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTTTGTCCCTTTGACTTGTAGTCC-3′

and

5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGAATGGGAAGGCATTTACG-3′

Off-target #1–2:

5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGAAACTAAGAGAGAAACGGCATG-3′

and

5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGAAATGTTTCAAGATGGGCCTC-3′

Off-target #1–3:

5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCAACCAATGGATCAGGACG-3′

and

5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCCTCAAAGAAAGGAACAACAGC-3′

Off-target #2–1:

5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGACAGGCATAAGACACCATACC-3′

and

5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCAAAGACCGGGTTCCAGATG-3′

Off-target #2–2:

5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGTCTGGGAAGAATAAGGGTATTGG-3′

and

5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTGGAGTCCACACCTGTTG-3′

Off-target #2–3:

5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAAGTGGGTCCTCAGTGAAAC-3′

and

5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTACCTTGCCTTCCTTCATGG-3′

HBc:

5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGACCGTTATAGAGTATTTGGT-3′

and

5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACAGGTACAGTAGAAGAAT-3′