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. 2017 Jul 21;7:6163. doi: 10.1038/s41598-017-06369-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of different coculture model systems. (A) The three different designs of coculture system, which were named after the relative interactions between CECs and LG cell spheroids. (B,C) Diffusion study (with fluorescent 10 kD dextran). Data in (C) was presented as percent of dextran diffused through. (D) LTF gene expression across the three coculture designs. (E) Secreted mucin layer from airlifted CECs in coculture, visualized by confocal microscopes (z scan). Scale bar 100 μm. (F) MUC5AC IHC staining (red fluorescence; nuclei were counterstained with DAPI) of the airlifted CECs. Scale bar 50 μm. *p < 0.05; **p < 0.001.