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. 2017 Jul 21;7:6134. doi: 10.1038/s41598-017-06463-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TNF-α and gap junction inhibitor induced actin rearrangement and focal adhesion formation. (A) HUVECs were treated with CBX and blebbistatin for 6 hours. The geometric means of the Young’s modulus of control group (n = 4), blebbistatin group (n = 5), CBX group (n = 5), and blebbistatin and CBX group (n = 5) were determined. Representative data from two experiments are shown. *P < 0.05, **P < 0.01; measured using a Tukey’s test followed by two-way ANOVA. (B) HUVECs were treated with CBX for 2 hours and then stimulated with TNF-α for 4 hours. F-actin (red) and nuclei (blue) were stained by rhodamine-phalloidin, and DAPI. Representative merged images from two experiments are shown. Scale bar showing 40 μm.