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. 2017 Jul 21;7:6145. doi: 10.1038/s41598-017-05504-9

Figure 1.

Figure 1

Generation of RTN1-null mice. (A) Schematic illustration of genomic structures of the mouse rtn1 gene, with each box representing an exon. The exon size is not proportionally represented. Exon 1A to 1F contains the translation initiation codon. The reticulon homology domain (RHD) is encoded by exons 4 to 9. (B) Targeting DNA vector (the standard Neo/TK cassette) is generated by insertion of two arms: one 5.4 kb fragment from intron 3, and the other 2.8 kd fragment having a sequence spanning intron 8 to exon 9. The restriction enzymes used for generation these two fragments are illustrated. The arrowhead indicates the Eco RI site. (C) Southern blotting was performed to validate the founder lines of RTN1 knockout (KO) mice and subsequently generated RTN1-null mice. KO mice produce an 11 kb DNA fragment, while wild-type (WT) mice produce a 6.4Kb DNA fragment.