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. 2017 Jul 21;7:6154. doi: 10.1038/s41598-017-06329-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analysis of PBP2A expression levels. Total protein extracts of COL, COL type IV, MW2 and their PBP4 mutants grown in the presence and absence of 0.5 µg/ml oxacillin (Oxa) were separated by 10% SDS-PAGE gel and assessed for PBP2A expression using anti-PBP2A antibody (top panel). The same PVDF membrane was probed using anti-mreC antibody as a loading control (bottom panel).