Figure 4.

Structured Illumination Microscopy (SIM) of COL and COL type IV in response to oxacillin challenge. COL and COL type IV were grown to early exponential phase before incubation for 1 h with oxacillin (256 µg/ml and 512 µg/ml, respectively). Cell were next incubated with Hoechst3332 (blue), Nile Red (red) and a fluorescent derivative of vancomycin (green) in order to stain the DNA, cell membrane and cell wall, respectively. Visualisation by SIM shows that the cell morphology of COL type IV is similar to COL, both in the presence and absence of oxacillin.