Table 1.
Characterization of dendrimer-based compounds.
| Compounds | y | M | MW (Da) | HD ± s.d. (nm) | PDI | ζ ± s.d. (mV) | ||
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| 1HNMR | MALDI | 1HNMR | MALDI | |||||
| G4OH | - | - | - | - | 14374 | 4.5 ± 1.9 | 0.121 | -0.1±0.4 |
| G4OH-yNH2 | 5.8 | 6.3 | - | - | 15091 | 4.1 ± 1.6 | 0.107 | +13.2±4.9 |
| Pep4 | - | - | - | 1363 | 9.6 ± 4.2 | 0.225 | +17.4±9.0 | |
| G4OH-mPep4 | 2.7* | 2.9** | 3.1 | 3.4 | 19723 | 6.1 ± 2.5 | 0.163 | +15.7±6.5 |
| G4OH+Pep4 | - | - | - | - | - | 5.4 ± 1.9 | 0.159 | +19.5±10.8 |
Note: Number of peripheral -NH2 groups of G4OH (y), number of Pep4 conjugated to G4OH (m), molecular weight (MW), hydrodynamic diameter (HD) and zeta potential of the dendrimers (G4OH and G4OH-yNH2), Pep4, G4OH-mPep4 conjugate and G4OH+Pep4 mixture as determined by 1HNMR, MALDI-TOF, and dynamic light scattering (DLS).
*
number of NH2 groups remaining after conjugation of Pep4 as determined by 1HNMR;
**
number of NH2 groups remaining after conjugation of Pep4 as determined by MALDI-TOF. PDI=polydispersity index.