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. Author manuscript; available in PMC: 2018 May 31.
Published in final edited form as: Vaccine. 2017 May 5;35(24):3239–3248. doi: 10.1016/j.vaccine.2017.04.062

Figure 2. Induction of PyCMP-Antigen-Specific T cells by SAd36PyCMP and Ad5PyCMP Immunization.

Figure 2

Female CB6F1/J mice (n = 5 per group) were immunized according to the regimens described in Table 1. PBMCs were obtained from mouse whole blood samples at days 10, 20, 40, 50, and 70-post priming and were processed for flow cytometry. (A) Kinetics of CD8+ T cells capable of recognizing the H-2Kd/SYVPSAEQI/APC tetramer induced over the course of the immunization regimen. (B) Kinetics of the percentage of CD4+ T cells expressing high levels of CD11a and CD49d, indicating antigen-experienced cells, in the course of the immunizations. (C) Average number of tetramer-specific CD8+ T cells induced by each immunization group were determined ten days after the final immunization (day 70). (D) Percentage of activated CD4+ T cells, determined by high levels of expression of CD49d and CD11a, at day 70, ten days after the final immunization. Results are presented as the percentages of positive cells per 106 PBMCs. Statistical analysis was conducted by Kruskal-Wallis test with Dunns post-test, statistically significant differences are denoted by *(p < 0.05) and ** (p < 0.01).